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AFLP (Amplified Fragment Length Polymorphism)

AFLP (Amplified Fragment Length Polymorphism) Also known as Selective Restriction Fragment Amplification (SRFA)  is a PCR-based technique developed in the early 1990’s by Keygene. AFLP is used in genetics research, DNA fingerprinting, and in the practice of genetic engineering to detect polymorphisms in DNA when no information about the genome is known. A DNA [...]

By | 2018-04-18T11:49:53+00:00 April 18th, 2018|Molecular Biology|Comments Off on AFLP (Amplified Fragment Length Polymorphism)

TCBS Agar

Thiosulfate-citrate-bile salts-sucrose agar or TCBS Agar is a selective media used for the isolation and cultivation of Vibrio cholerae and other enteropathogenic Vibrio's causing food poisoning Principle TCBS Agar was developed by Kobayashi and co-workers who modified the selective medium of Nakanishi. TCBS agar was originally designed for the isolation of V.cholerae and V. parahaemolyticus [...]

By | 2018-04-18T11:44:25+00:00 April 18th, 2018|Food Microbiology|Comments Off on TCBS Agar

Oxidase and Indole test

Oxidase Test The oxidase test is performed to identify the bacteria if it produces cytochrome c oxidase and to detect the presence of an enzyme called cytochrome c oxidase that catalyses the transport of electrons between electron donors that are present in the bacteria and a redox dye- tetramethyl-p-phenylene-diamine. In case of the positive result [...]

By | 2018-04-18T10:38:59+00:00 April 18th, 2018|Food Microbiology|Comments Off on Oxidase and Indole test

Gene cloning

Gene cloning is molecular technique in which gene of interest is copied to produced many identical copies of it. In this technique gene of interest is fused into a self-replicating genetic material i.e. plasmid. The insertion of gene of interest is done using self enzymes known as restriction enzymes. The molecule formed after the [...]

By | 2018-04-18T09:59:18+00:00 April 18th, 2018|Molecular Biology|Comments Off on Gene cloning

Plasmid vectors

Vectors can be described as an autonomously replicating DNA sequence that can be used to carry foreign DNA fragments. Commonly used vectors are bacteriophages and plasmids. A vector that is used predominantly for reproducing DNA segment is referred to as cloning vectors and vectors used for expressing a gene contained within the cloned DNA is [...]

By | 2018-04-17T12:21:29+00:00 April 17th, 2018|Molecular Biology|Comments Off on Plasmid vectors

Culture Media for Plant tissue culture

All tissue culture media are mostly synthetic or chemically defined. A few of them have organic complex nutrients e.g., potato extract. Although, a variety of media have been formulated to date but none of them is suitable for all plant species. The composition of White, MS and B5, the most commonly used media are listed [...]

By | 2018-04-17T12:18:41+00:00 April 17th, 2018|Plant Tissue Culture|Comments Off on Culture Media for Plant tissue culture

Laminar air flow

Laminar air flow is an enclosed bench designed to maintain a working area devoid of contaminants. Many medical, microbiological and research laboratories require aseptic working environments in order to carry out specialized work. Laminar air Flow can provide the best solution. Laminar Flow Cabinets or laminar air flow is normally made of stainless steel without [...]

By | 2018-04-17T10:00:22+00:00 April 17th, 2018|Fermentation, Food Microbiology, Molecular Biology, Plant Tissue Culture|Comments Off on Laminar air flow

Probiotics

Probiotics are the live bacteria and yeasts that are referred to as the good microorganisms that are required in the body because they are beneficial to us as they aid us with the improved digestive system. Probiotics are marketed in forms of food, dietary supplements for humans and for animals as well. These are the [...]

By | 2018-04-17T09:40:58+00:00 April 17th, 2018|Food Microbiology|Comments Off on Probiotics

Biosafety Levels

Biosafety levels Biosafety levels are process of keeping the harmful biological agents under control. It includes the precautions required to isolate dangerous microbes in as laboratory. There are four biosafety levels are designed from lowest biosafety to highest biosafety i.e. BSL 1 to BSL 4. The precautions level consist of regular and washing and [...]

By | 2018-04-19T06:33:51+00:00 April 17th, 2018|Food Microbiology|Comments Off on Biosafety Levels

Biochemical tests

Biochemical tests are the tests that are used for the identification or confirmation of different bacterial species or different micro organisms based on the biochemical activities that the microorganisms are performing. In order to classify different bacteria as unique or different from each other we need to identify them as unique and to do their [...]

By | 2018-04-16T12:07:57+00:00 April 16th, 2018|Food Microbiology|Comments Off on Biochemical tests

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