Molecular Biology

/Molecular Biology

The Electrocardiograph (ECG)

The principle and significance: The Electrocardiograph (ECG): It is clinically very useful, as it shows the electrical activity within the heart, simply by placing electrodes at various points on the body surface. This enables clinicians to determine the state of the conducting system and of the myocardium itself, as damage to the myocardium alters the [...]

By | 2018-05-12T11:16:41+00:00 May 12th, 2018|Molecular Biology|Comments Off on The Electrocardiograph (ECG)

Light signaling

Light is the most important source of energy for plants for continuous uptake of energy and for themselves to the changes in their natural environment because they cannot move and change their position. So in order to regulate their photosynthetic capacity in view of the changing light environment they have to adapt themselves. For [...]

By | 2018-05-12T10:50:29+00:00 May 12th, 2018|Molecular Biology|Comments Off on Light signaling

Cell signalling

Cells usually do not live in isolation. Cells lie in the form of groups in diverse tissues and cells. Therefore, cell to cell communication network is needed to coordinate the growth, differentiation and metabolism of the cells. Direct cell to cell contact is a way of communication in cells present in small groups. But [...]

By | 2018-05-11T10:56:03+00:00 May 11th, 2018|Food Microbiology, Molecular Biology|Comments Off on Cell signalling

Isolation of specific nucleic acid sequences

Polymerase Chain Reaction (PCR) The polymerase chain reaction technique is carried out in vitro and is used for the amplification of DNA. It was developed by Kary Mullis in 1983. Through this technique billion copies of the desired DNA or RNA can be made in a matter of few hours. The PCR reaction mix contains [...]

By | 2018-05-08T09:55:35+00:00 May 8th, 2018|Molecular Biology|Comments Off on Isolation of specific nucleic acid sequences

Sex linkage

Sex chromosomes Apart from 22 paired chromosomes in humans which are known as autosomes there is one set of chromosomes in both males and females that are known as sex chromosome. This is called so because it determines sex of the person. In females all chromosomes are in homologous pair-XX and in male‘s heterozygous pair [...]

By | 2018-05-05T11:37:14+00:00 May 5th, 2018|Molecular Biology|Comments Off on Sex linkage

Somatic cell Hyberdization – Fusion of Protoplasts

Somatic cell hyberdization is a type of genetic modification in plants. In this process  a new hybrid plant produced  after the fusion of  two distinct species of plants. A new hybrid species   produced consist of characteristic of both. Hybrids have been produced either between different varieties of the same species or between two different species. [...]

By | 2018-05-04T12:11:52+00:00 May 4th, 2018|Molecular Biology|Comments Off on Somatic cell Hyberdization – Fusion of Protoplasts

Genome Sequencing – High Throughput Sequencing

High throughput sequencing technologies play major role in studies on genomics, epigenomics, and transcriptomics. This technology is capable of sequencing multiple DNA molecules in parallel, enabling hundreds of millions of DNA molecules to be sequenced at a time. This advantage allows high throughput sequencing to be used to create large data sets, generating more [...]

By | 2018-05-04T05:27:51+00:00 May 3rd, 2018|Molecular Biology|Comments Off on Genome Sequencing – High Throughput Sequencing

Genome Sequencing – Chain Termination Method

DNA sequencing is the process of determining the sequence of nucleotides within a DNA molecule. It includes technology that is used to determine the order of the four bases: adenine, guanine, cytosine and thymine —in a strand of DNA. Method of Sequencing: Sanger sequencing This method is also known as the chain termination method. [...]

By | 2018-05-04T05:29:08+00:00 May 3rd, 2018|Molecular Biology|Comments Off on Genome Sequencing – Chain Termination Method

Restriction Fragment Length Polymorphism

RFLP is technique exploits separation and identification of desired fragments of DNA. It is used to identify variations in homologous DNA sequences. This technique is invented by Alec Jeffreys in 1984. Genetic polymorphism is defined as the inherited genetic differences among individuals in over 1% of normal population. The RFLP technique exploits these differences [...]

By | 2018-05-04T05:30:27+00:00 May 2nd, 2018|Molecular Biology|Comments Off on Restriction Fragment Length Polymorphism

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