Restriction Fragment Length Polymorphism
Restriction fragment length polymorphism (RFLP) method in molecular biology was evolved for detecting variation at the DNA sequence level of various biological samples. The principle of this method is based upon the comparison of restriction enzyme cleavage profiles following the existence of a polymorphism in a DNA sequence related to other sequence. In RFLP, DNA of individuals to be comparedis digested with one or more restriction enzymes and the resulting fragments are separated according to molecular size using gel electrophoresis along with a molecular weight marker. Through this approach two individuals can present different restriction profiles.
Restriction fragment length polymorphism (RFLP) analysis is extensively used in molecular biology for detecting variation at the DNA sequence level. The principle of this analysis is to compare restriction digestion profiles of DNA samples isolated from different individuals. RFLP functions as a molecular marker as it is specific to a single clone/restriction enzyme combination.Most RFLP markers are co-dominant and highly locus-specific. In molecular biology, restriction fragment length polymorphism, or RFLP is a technique that exploits variations in homologous DNA sequences. It refers to a difference between samples of homologousDNA molecules that come from differing locations of restriction enzyme sites, and to a related laboratory technique by which these segments can be illustrated.
RFLP is a difference in homologous DNA sequences that can be detected by the presence of fragments of different lengths after digestion of the DNA samples in question with specific restriction endonucleases. The basic technique for detecting RFLPs involves fragmenting a sample of DNA by a restriction enzyme, which can recognize and digest DNA wherever a specific short sequence occurs, in a process known as restriction digestion. The resulting DNA fragments are then separated by length through a process known as agarose gel electrophoresis. Molecular markers are used to estimate the fragment size. RFLP is specific to a single clone/restriction enzyme combination and it occurs when the length of a detected fragment varies between individuals.