plant tissue culture

/Tag: plant tissue culture

Organogenesis in plants

Organization of shoot apical meristem (SAM): These are the small living cells, which retain the capacity of cell division. Shoot apical meristem has embryonic characteristics. The shoot apical meristem is the functional unit of the shoot apex, it consists of apical meristem along with primordia. During differentiation of leaf primordial, shoot axillary meristem develops from [...]

By | 2018-05-02T12:03:06+00:00 May 2nd, 2018|Plant Tissue Culture|Comments Off on Organogenesis in plants

Plant tissue culture media

Growth and morphogenesis of plant in vitro condition require proper composition of culture media. The culture media could be solid media or liquid media. The discovery of IAA by Went and kinetin by Skoog sparks the discovery of culture media. Skoog and Miller in 1957 demonstrated that totipotency and regulation of morphogenesis is through the [...]

By | 2018-04-28T11:48:53+00:00 April 28th, 2018|Plant Tissue Culture|Comments Off on Plant tissue culture media

Culture Media for Plant tissue culture

All tissue culture media are mostly synthetic or chemically defined. A few of them have organic complex nutrients e.g., potato extract. Although, a variety of media have been formulated to date but none of them is suitable for all plant species. The composition of White, MS and B5, the most commonly used media are listed [...]

By | 2018-04-17T12:18:41+00:00 April 17th, 2018|Plant Tissue Culture|Comments Off on Culture Media for Plant tissue culture

Abscisic acid (ABA)

It is synthesized in plastids from carotenoids and diffuses in all directions through vascular tissues and parenchyma. The transport of ABA can occur in both xylem and phloem tissues. It can also be translocated through parenchyma cells. The movement of abscisic acid in plants does not exhibit polarity like auxins. ABA is capable of [...]

By | 2018-04-04T12:13:06+00:00 April 4th, 2018|Plant Tissue Culture|Comments Off on Abscisic acid (ABA)

Scope and Training of Plant Tissue Culture

Plant tissue culture broadly refers to the in vitro cultivation of plants, seeds and various parts of the plants (organs, embryos, tissues, single cells, protoplasts). Plant tissue culture is one of the most rapidly growing areas of biotechnology because of its high potential to develop improved crops and ornamental plants. With the advances made [...]

By | 2018-03-26T12:13:28+00:00 March 5th, 2018|Plant Tissue Culture|Comments Off on Scope and Training of Plant Tissue Culture

Steps in plant tissue culture

STAGE 1: Initiation phase The initiation phase is the first phase of tissue culture. Here, the tissue of interest is obtained and introduced and sterilized in order to prevent any microorganism from negatively affecting the process. It is during this stage that the tissue is initiated in to culture. STAGE 2: Multiplication stage The [...]

By | 2018-02-27T08:53:32+00:00 February 21st, 2018|Plant Tissue Culture|Comments Off on Steps in plant tissue culture

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