Microscopic technique – application of neubaur chamber for cell count (lecocyte cell concentration)
The number of leukocytes contained in 1 litre of blood is called the leukocyte number concentration or leukocyte count. In certain diseases the number of leukocytes in the blood is altered. For example, in infectious mononucleosis and bacterial infections there is a marked increase, whereas in typhoid fever there is a marked decrease. The blood is diluted in a leukocyte diluting fluid which haemolyses the erythrocytes, but leaves the leukocytes intact. The leukocytes are then counted in a counting chamber under the microscope, and the number of cells per litre of blood is calculated.