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So far obpl has created 167 blog entries.

Plant development

Arabidopsis thaliana is the developmental model organism. In plants, there is no special germ line in the embryo and germ cells arise from somatic cells during post-embryonic development, although they all appear to arrive from single cell layer. The plant embryo compromises two organ systems Embryonic axis: It gives rise to seedling. Cotyledons, either [...]

By |2018-05-04T04:30:56+00:00May 3rd, 2018|Plant Tissue Culture|Comments Off on Plant development

Restriction Fragment Length Polymorphism

RFLP is technique exploits separation and identification of desired fragments of DNA. It is used to identify variations in homologous DNA sequences. This technique is invented by Alec Jeffreys in 1984. Genetic polymorphism is defined as the inherited genetic differences among individuals in over 1% of normal population. The RFLP technique exploits these differences [...]

By |2018-05-04T05:30:27+00:00May 2nd, 2018|Molecular Biology|Comments Off on Restriction Fragment Length Polymorphism

Organogenesis in plants

Organization of shoot apical meristem (SAM): These are the small living cells, which retain the capacity of cell division. Shoot apical meristem has embryonic characteristics. The shoot apical meristem is the functional unit of the shoot apex, it consists of apical meristem along with primordia. During differentiation of leaf primordial, shoot axillary meristem develops from [...]

By |2018-05-02T12:03:06+00:00May 2nd, 2018|Plant Tissue Culture|Comments Off on Organogenesis in plants

Mode of Transport across Plasma Membrane: Passive transport

  The plasma membrane acts as a semi-permeable barrier between the cell and the extracellular environment. This permeability must be highly selective to ensure that essential molecules such as glucose, amino acids and lipids can readily enter the cell so that these molecules and metabolic intermediates remain in the cell and waste compounds leave the [...]

By |2018-05-01T11:52:27+00:00May 1st, 2018|Plant Tissue Culture|Comments Off on Mode of Transport across Plasma Membrane: Passive transport

Gene Regulation in Prokaryotes

Gene regulation in prokaryotes is one of the method of conservation of cell resources by turning OFF and ON of genes transcribing. Controlling of gene expression leads to regulation of metabolism of cell. Regulations of prokaryotic genes are done in units called as Operons. Operons are genes grouped together translated into single mRNA molecule containing [...]

By |2018-05-01T11:16:22+00:00May 1st, 2018|Food Microbiology, Molecular Biology|Comments Off on Gene Regulation in Prokaryotes

DNA Polymerase

DNA polymerases DNA polymerase enzyme that synthesizes DNA by assembling nucleotides. DNA polymerase was purified by Arthur Kornberg in 1955 from E. coli cells now known as DNA polymerase I In 1959; Kornberg was awarded with Noble Prize in physiology and medicine. DNA synthesis occurs by phosphoryl group transfer. 3ˈ-OH group is nucleophile and [...]

By |2018-05-01T03:59:14+00:00April 30th, 2018|Molecular Biology|Comments Off on DNA Polymerase

Viability Assay – MTT Assay

Viability of cell is its ability to grow or stay alive. The viability assay is to check how many cells in culture are viable. Determination  of  the viability of cultured cells is important step prior to any cell-based research because only a healthy culture will show reliable and reproducible results. Viability assay helps to [...]

By |2018-05-04T06:01:46+00:00April 30th, 2018|Molecular Biology|Comments Off on Viability Assay – MTT Assay

Viruses

Viruses are obligate parasites. The field of study in which viruses are investigated is called virology. Viruses are the agents considered on the borderline of living organisms, come in various shapes, and are widely distributed in nature, infecting animal cells, plant cells, and microorganisms also. Once a virus infects a host cell it can direct [...]

By |2018-04-30T09:53:43+00:00April 30th, 2018|Food Microbiology, Molecular Biology|Comments Off on Viruses

Plant tissue culture media

Growth and morphogenesis of plant in vitro condition require proper composition of culture media. The culture media could be solid media or liquid media. The discovery of IAA by Went and kinetin by Skoog sparks the discovery of culture media. Skoog and Miller in 1957 demonstrated that totipotency and regulation of morphogenesis is through the [...]

By |2018-04-28T11:48:53+00:00April 28th, 2018|Plant Tissue Culture|Comments Off on Plant tissue culture media

News Updates

  • An MoU was signed between Orbit Biotech and Govt of Punjab during the Progressive Punjab Invest Summit 2015.
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