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So far obpl has created 167 blog entries.

Gene Therapy

Gene therapy is when DNA is introduced into a patient to treat a genetic disease. The new DNA usually contains a functioning gene to correct the effects of a disease-causing mutation. The process of gene therapy includes many approaches like: Replacing of mutated gene (work incorrectly or no longer work at all) with copy [...]

By |2018-04-28T07:31:30+00:00April 28th, 2018|Molecular Biology|Comments Off on Gene Therapy

Flow Cytometry

Flow cytometry is a molecular technique that is used to analyze the physical and chemical characteristics of particles in a fluid as it passes through  laser. It is laser-based technology used to count, sort, and profile cells in a heterogeneous fluid mixture. In this technique the cell components are fluorescently labeled and then excited by [...]

By |2018-04-20T12:02:46+00:00April 20th, 2018|Molecular Biology|Comments Off on Flow Cytometry

Active transport

Active transport uses specific transport proteins, called pumps, which use metabolic energy (ATP) to move ions or molecules against their concentration gradient. For example, in both vertebrates and invertebrates, the concentration of sodium ion is about 10 to 20 times higher in the blood than within the cell. The concentration of the potassium ion is [...]

By |2018-04-20T11:49:53+00:00April 20th, 2018|Food Microbiology, Molecular Biology|Comments Off on Active transport

Plasma membrane: Bulk transport

Cells can import and export large molecules across the plasma membrane. Macromolecules are secreted out from the cell by exocytosis and are ingested into the cell from outside through phagocytosis and endocytosis. Exocytosis It is also called emeiocytosis and cell vomiting. In all eukaryotic cells, secretary vesicles are continually carrying new plasma membrane and cellular [...]

By |2018-04-20T11:26:22+00:00April 20th, 2018|Food Microbiology, Molecular Biology|Comments Off on Plasma membrane: Bulk transport

M. tuberculosis Culture

Aerobic conditions are required for the growth of M. tuberculosis and a protein enriched medium is a prerequisite for its culture. Löwenstein–Jensen (LJ) slopes are main agar media employed to culture Mycobacteria. It composition is: Inspissated eggs Malachite green Glycerol (or pyruvate). Glycerol in the LJ medium favours the growth of M. tuberculosis while [...]

By |2018-04-19T11:51:27+00:00April 19th, 2018|Food Microbiology|Comments Off on M. tuberculosis Culture

Microbial Interactions

Microbial Interactions The population of two species may interact for neutral, positive and negative represented as 00, - -, + +, + 0, - 0 and + - . There are mainly nine types of interactions based on whether species are not gaining, gaining or harming to the other species in a given relationship. [...]

By |2018-04-19T11:45:41+00:00April 19th, 2018|Food Microbiology|Comments Off on Microbial Interactions

Mechanisms of Action of Probiotics

There are many microorganisms that could potentially function as probiotic, of which Lactobacillus and Bifidobacterium species are the most commonly used. Probiotics are live microorganisms thought to be beneficial to the host organism. According to the definition of FAO/WHO, probiotics are live microorganisms, which when administered in adequate amounts confer a health benefit on [...]

By |2018-04-19T11:11:39+00:00April 19th, 2018|Food Microbiology|Comments Off on Mechanisms of Action of Probiotics

RNA Splicing

The coding sequence of a gene is a series of three nucleotide codons that specify a sequence of amino acids in its polypeptide product. The codon for each amino acid is immediate adjacent of the codon of the next amino acid in a polypeptide chain in case of prokaryotic genome. Eukaryotic genome is composed [...]

By |2018-04-19T11:10:23+00:00April 19th, 2018|Molecular Biology|Comments Off on RNA Splicing

Transformation

Transformation is the process of transfer of exogenous genetic material into the cell through the cell membranes resulting in alteration of genetic material of host cell. The transformation process required the recipient bacteria must be in a state of competence i.e. state of being able to take up exogenous material from the environment, which [...]

By |2018-04-19T09:13:10+00:00April 19th, 2018|Molecular Biology|Comments Off on Transformation

Chemostat (A Continous Culture)

A chemostat is a technique used to grow microorganisms or cells continually in a specific phase of growth. In this technique a bioreactor is provided with fresh medium which is continuously added, and culture liquid containing left over nutrients, metabolic end products and to keep the culture volume constant microorganisms are continuously removed at the [...]

By |2018-04-19T06:39:55+00:00April 18th, 2018|Fermentation|Comments Off on Chemostat (A Continous Culture)

News Updates

  • An MoU was signed between Orbit Biotech and Govt of Punjab during the Progressive Punjab Invest Summit 2015.
  • Registrations open for the biotech industrial training program starting April/May/June/July 2019. For registrations contact us at +91. 85 91 87 1105