Characterisation by motility testing: Cragie’s tube method

/Characterisation by motility testing: Cragie’s tube method
Characterisation by motility testing: Cragie’s tube method2018-02-26T11:28:48+00:00

Characterisation by motility testing: Cragie’s tube method

Characterisation by motility testing: Hanging drop method, Cragie’s tube method, U-tube method and Wet mount

Introduction

Cragie’s tube is the normal tube filled with semi solid agar containing a capillary inside it. The bacterial culture is inoculated into the capillary. Motile bacteria will come outside the capillary from the bottom and grow in the agar inside the outer tube whereas the non-motile bacteria will remain inside the capillary.

Requirements

  1. Bacterial culture
  2. Cragie’s tube filled with semi solid agar
  3. Inoculating loop (straight wire)

Procedure

  1. Inoculate the 24 hrs bacterial culture into capillary tube.
  2. Incubate the Cragie’s tube in upright position overnight.
  3. Next day observe the tube for growth of bacteria on outside of the capillary.
  4. Interpret the results in the following manner.
  5. Growth on outside of the capillary – motile bacteria
  6. No growth on outside of the capillary – non-motile bacteria
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