DNA

/Tag: DNA

Genome Sequencing – High Throughput Sequencing

High throughput sequencing technologies play major role in studies on genomics, epigenomics, and transcriptomics. This technology is capable of sequencing multiple DNA molecules in parallel, enabling hundreds of millions of DNA molecules to be sequenced at a time. This advantage allows high throughput sequencing to be used to create large data sets, generating more [...]

By | 2018-05-04T05:27:51+00:00 May 3rd, 2018|Molecular Biology|Comments Off on Genome Sequencing – High Throughput Sequencing

Genome Sequencing – Chain Termination Method

DNA sequencing is the process of determining the sequence of nucleotides within a DNA molecule. It includes technology that is used to determine the order of the four bases: adenine, guanine, cytosine and thymine —in a strand of DNA. Method of Sequencing: Sanger sequencing This method is also known as the chain termination method. [...]

By | 2018-05-04T05:29:08+00:00 May 3rd, 2018|Molecular Biology|Comments Off on Genome Sequencing – Chain Termination Method

Restriction Fragment Length Polymorphism

RFLP is technique exploits separation and identification of desired fragments of DNA. It is used to identify variations in homologous DNA sequences. This technique is invented by Alec Jeffreys in 1984. Genetic polymorphism is defined as the inherited genetic differences among individuals in over 1% of normal population. The RFLP technique exploits these differences [...]

By | 2018-05-04T05:30:27+00:00 May 2nd, 2018|Molecular Biology|Comments Off on Restriction Fragment Length Polymorphism

Gene Therapy

Gene therapy is when DNA is introduced into a patient to treat a genetic disease. The new DNA usually contains a functioning gene to correct the effects of a disease-causing mutation. The process of gene therapy includes many approaches like: Replacing of mutated gene (work incorrectly or no longer work at all) with copy [...]

By | 2018-04-28T07:31:30+00:00 April 28th, 2018|Molecular Biology|Comments Off on Gene Therapy

Transformation

Transformation is the process of transfer of exogenous genetic material into the cell through the cell membranes resulting in alteration of genetic material of host cell. The transformation process required the recipient bacteria must be in a state of competence i.e. state of being able to take up exogenous material from the environment, which [...]

By | 2018-04-19T09:13:10+00:00 April 19th, 2018|Molecular Biology|Comments Off on Transformation

Analysis of RNA, DNA and proteins by one and two-dimensional gel electrophoresis

Agarose gel electrophoresis Agarose gel electrophoresis is the common way of separating and analyzing DNA biomolecules such as DNA and RNA. It is an analytical method for determining the size of DNA molecules in the range of 500 to 30,000 base pairs. DNA bands visualization can be done by the addition of Ethidium bromide in [...]

By | 2018-03-19T03:46:52+00:00 March 19th, 2018|Molecular Biology|Comments Off on Analysis of RNA, DNA and proteins by one and two-dimensional gel electrophoresis

Molecular cloning

Molecular cloning of desired gene in bacterial and eukaryotic systems and expression of recombinant proteins using different vectors: Vectors Vectors can be described as an autonomously replicating DNA sequence that can be used to carry foreign DNA fragments. Commonly used vectors are bacteriophages and plasmids. Insertion λ vectors are used for constructing cDNA libraries. [...]

By | 2018-03-16T16:59:11+00:00 March 16th, 2018|Molecular Biology|Comments Off on Molecular cloning

Isolation and purification of RNA and DNA

Isolation and purification of nucleic acids are very important for any molecular biology experiment. It is the starting material for all types of molecular biology experiments like restriction digestion, cloning, PCR, DNA-libraries and sequencing. By manipulating this extracted DNA or RNA, a new DNA with different characteristics can be obtained. Genetic engineering process relies [...]

By | 2018-03-15T11:18:31+00:00 March 15th, 2018|Molecular Biology|Comments Off on Isolation and purification of RNA and DNA

Benefits of molecular biology training

Molecular biology pertains to the study of living systems at the DNA, RNA, and protein levels, and how these molecules can be modified and harnessed for practical applications. Knowledge of the natural function of these molecules in the cell provides a context appropriate for further advancement in the rapidly expanding areas of functional genomics, [...]

By | 2018-02-27T07:09:47+00:00 February 21st, 2018|Molecular Biology|Comments Off on Benefits of molecular biology training

News Updates

  • An MoU was signed between Orbit Biotech and Govt of Punjab during the Progressive Punjab Invest Summit 2015.
  • Registrations open for the biotech industrial training program starting April/May/June/July 2018. For registrations contact us at +91. 85 91 87 1105