plasmid

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Gene cloning

Gene cloning is molecular technique in which gene of interest is copied to produced many identical copies of it. In this technique gene of interest is fused into a self-replicating genetic material i.e. plasmid. The insertion of gene of interest is done using self enzymes known as restriction enzymes. The molecule formed after the [...]

By |2018-04-18T09:59:18+00:00April 18th, 2018|Molecular Biology|Comments Off on Gene cloning

Plasmid vectors

Vectors can be described as an autonomously replicating DNA sequence that can be used to carry foreign DNA fragments. Commonly used vectors are bacteriophages and plasmids. A vector that is used predominantly for reproducing DNA segment is referred to as cloning vectors and vectors used for expressing a gene contained within the cloned DNA is [...]

By |2018-04-17T12:21:29+00:00April 17th, 2018|Molecular Biology|Comments Off on Plasmid vectors

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