/Tag: Probe

Southern blotting

Southern blotting is an example of RFLP (restriction fragment length polymorphism). It was developed by Edward M. Southern (1975). Southern blotting is a hybridization technique for identification of particular size of DNA from the mixture of other similar molecules. This technique is based on the principle of separation of DNA [...]

By |2018-02-27T09:08:53+00:00February 20th, 2018|Molecular Biology|Comments Off on Southern blotting


Blotting means to transfer DNA or RNA to nitrocellulose membrane and probe binds to complementary strand in the sample to give result in the form of signal using radioactive or biotin labelled probe. Principle At high temperature two strands of DNA get separated they and they re-anneal when temperature is brought back to normal. [...]

By |2018-02-27T08:53:08+00:00February 20th, 2018|Molecular Biology|Comments Off on Blotting

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