RNA

/Tag: RNA

Ribonucleic acid (RNA)

The backbone of RNA is composed of ribose rather than 2ˈ-deoxyribose. That is, it has a hydroxyl group at its 2ˈ position. RNA has uracil in place of thymine. Uracil lacks the 5ˈ methyl group which is present in thymine. RNA is a single polynucleotide chain. RNA functions as an intermediate (the mRNA) between the [...]

By |2018-04-06T04:00:44+00:00April 5th, 2018|Molecular Biology|0 Comments

Structure of RNA

The backbone of RNA is composed of ribose rather than 2ˈ-deoxyribose. That is, it has a hydroxyl group at its 2ˈ position. RNA has uracil in place of thymine. Uracil lacks the 5ˈ methyl group which is present in thymine. RNA is a single polynucleotide chain. RNA functions as an intermediate (the mRNA) between [...]

By |2018-03-27T11:28:08+00:00March 27th, 2018|Molecular Biology|Comments Off on Structure of RNA

Analysis of RNA, DNA and proteins by one and two-dimensional gel electrophoresis

Agarose gel electrophoresis Agarose gel electrophoresis is the common way of separating and analyzing DNA biomolecules such as DNA and RNA. It is an analytical method for determining the size of DNA molecules in the range of 500 to 30,000 base pairs. DNA bands visualization can be done by the addition of Ethidium bromide in [...]

By |2018-03-19T03:46:52+00:00March 19th, 2018|Molecular Biology|Comments Off on Analysis of RNA, DNA and proteins by one and two-dimensional gel electrophoresis

Molecular cloning

Molecular cloning of desired gene in bacterial and eukaryotic systems and expression of recombinant proteins using different vectors: Vectors Vectors can be described as an autonomously replicating DNA sequence that can be used to carry foreign DNA fragments. Commonly used vectors are bacteriophages and plasmids. Insertion λ vectors are used for constructing cDNA libraries. [...]

By |2018-03-16T16:59:11+00:00March 16th, 2018|Molecular Biology|Comments Off on Molecular cloning

Isolation and purification of RNA and DNA

Isolation and purification of nucleic acids are very important for any molecular biology experiment. It is the starting material for all types of molecular biology experiments like restriction digestion, cloning, PCR, DNA-libraries and sequencing. By manipulating this extracted DNA or RNA, a new DNA with different characteristics can be obtained. Genetic engineering process relies [...]

By |2018-03-15T11:18:31+00:00March 15th, 2018|Molecular Biology|Comments Off on Isolation and purification of RNA and DNA

RNA Extraction

RNA (Ribonucleic acid) is a polymeric substance present in living cells and many viruses, consisting of a long single-stranded chain of phosphate and ribose units with the nitrogen bases adenine, guanine, cytosine, and uracil, which are bonded to the ribose sugar. RNA is used in all the steps of protein synthesis in all living cells [...]

By |2018-02-27T09:01:57+00:00February 20th, 2018|Molecular Biology|Comments Off on RNA Extraction

DNA Microarray

The microarray is a recently developed technology used in cancer research and in the pharmacological treatment of other diseases such as oral lesions. In this technology, a microscope slide (normally a 2D array made of glass, silicon chips, or nylon membrane) printed with thousands of minute spots in definite positions is used. DNA microarray [...]

By |2018-02-27T09:06:30+00:00February 20th, 2018|Molecular Biology|Comments Off on DNA Microarray

Gene Cloning: A breakthrough

During the last few decades, techniques for manipulating eukaryotic as well as prokaryotic DNA have witnessed a remarkable development. There are three main phases of the development of these techniques, which include recombinant DNA technology and gene cloning; polymerase chain reaction and DNA chips and microarrays. Using recombinant DNA technology, we can isolate and [...]

By |2018-02-27T08:34:22+00:00December 19th, 2016|Molecular Biology|Comments Off on Gene Cloning: A breakthrough

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