/Tag: SDS

Isolation and purification of RNA and DNA

Isolation and purification of nucleic acids are very important for any molecular biology experiment. It is the starting material for all types of molecular biology experiments like restriction digestion, cloning, PCR, DNA-libraries and sequencing. By manipulating this extracted DNA or RNA, a new DNA with different characteristics can be obtained. Genetic engineering process relies [...]

By |2018-03-15T11:18:31+00:00March 15th, 2018|Molecular Biology|Comments Off on Isolation and purification of RNA and DNA

RNA Extraction

RNA (Ribonucleic acid) is a polymeric substance present in living cells and many viruses, consisting of a long single-stranded chain of phosphate and ribose units with the nitrogen bases adenine, guanine, cytosine, and uracil, which are bonded to the ribose sugar. RNA is used in all the steps of protein synthesis in all living cells [...]

By |2018-02-27T09:01:57+00:00February 20th, 2018|Molecular Biology|Comments Off on RNA Extraction

Southern blotting

Southern blotting is an example of RFLP (restriction fragment length polymorphism). It was developed by Edward M. Southern (1975). Southern blotting is a hybridization technique for identification of particular size of DNA from the mixture of other similar molecules. This technique is based on the principle of separation of DNA [...]

By |2018-02-27T09:08:53+00:00February 20th, 2018|Molecular Biology|Comments Off on Southern blotting

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