/Tag: Tissue

Plant shoot tip preservation

The cryopreservation of plant cell culture followed by the regeneration of plants broadly involves the following stages 1. Development of sterile tissue cultures 2. Addition of cryoprotectants and pretreatment 3. Freezing 4. Storage 5. Thawing 6. Re-culture 7. Measurement of survival/viability 8. Plant regeneration. Development of sterile tissue culture: The selection of plant species [...]

By |2018-05-08T04:06:46+00:00May 7th, 2018|Plant Tissue Culture|Comments Off on Plant shoot tip preservation

Protoplast Isolation

The term protoplast was introduced by Hanstein in 1880. It refers to the cellular content excluding cell wall or can also be called as a naked plant cell. It is described as living matter enclosed by a plant cell membrane. Protoplast isolation for the first time was carried out by Klercker in 1892 using the [...]

By |2018-03-26T12:12:23+00:00February 21st, 2018|Plant Tissue Culture|Comments Off on Protoplast Isolation

Benefits of plant tissue culture training

Plant tissue culture broadly refers to the in vitro cultivation of plants, seeds and various parts of the plants (organs, embryos, tissues, single cells, protoplasts). The cultivation process is invariably carried out in a nutrient culture medium under aseptic conditions Plant tissue culture is one of the most rapidly growing areas of biotechnology because [...]

By |2018-02-27T06:50:37+00:00February 21st, 2018|Plant Tissue Culture|Comments Off on Benefits of plant tissue culture training


Cryopreservation means preservation in the frozen state. The principle involved in cryopreservation is to bring the plant cell and tissue cultures to a zero metabolism or non-dividing state by reducing the temperature in the presence of cryoprotectants. Cryopreservation broadly means the storage of germplasm at very low temperatures: Over solid carbon dioxide (at -79°C) [...]

By |2018-05-07T12:07:51+00:00February 20th, 2018|Plant Tissue Culture|Comments Off on Cryopreservation

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