PCR

/Tag: PCR

Isolation of specific nucleic acid sequences

Polymerase Chain Reaction (PCR) The polymerase chain reaction technique is carried out in vitro and is used for the amplification of DNA. It was developed by Kary Mullis in 1983. Through this technique billion copies of the desired DNA or RNA can be made in a matter of few hours. The PCR reaction mix contains [...]

By |2018-05-08T09:55:35+00:00May 8th, 2018|Molecular Biology|Comments Off on Isolation of specific nucleic acid sequences

Genome Sequencing – High Throughput Sequencing

High throughput sequencing technologies play major role in studies on genomics, epigenomics, and transcriptomics. This technology is capable of sequencing multiple DNA molecules in parallel, enabling hundreds of millions of DNA molecules to be sequenced at a time. This advantage allows high throughput sequencing to be used to create large data sets, generating more [...]

By |2018-05-04T05:27:51+00:00May 3rd, 2018|Molecular Biology|Comments Off on Genome Sequencing – High Throughput Sequencing

Types of PCR

Inverse PCR Inverse PCR can be used to amplify the sequences flanking a segment of the border sequences which are known. This is done by using prime oligonucleotides complementary to the 5‘ends of the desired segment. This orients the free 3‘OH of the primers outward of the sequence, as a result the newly synthesized [...]

By |2018-04-13T12:01:53+00:00April 12th, 2018|Molecular Biology|Comments Off on Types of PCR

Molecular cloning

Molecular cloning is the process of inserting a DNA sequence (generally a gene) from a selected species into a replicating vehicles like vectors or plasmids, for propagation, without any alteration or modification of the original DNA sequence. Molecular clones can be used to express the resulting proteins for the utilization of protein’s functions. It [...]

By |2018-02-27T07:53:28+00:00February 21st, 2018|Molecular Biology|Comments Off on Molecular cloning

Applications of Polymerase Chain Reaction (PCR)

The polymerase chain reaction technique is carried out in vitro and is used for the amplification of DNA. It was developed by Kary Mullis in 1983. Through this technique a billion copies of the desired DNA or RNA can be made in a matter of few hours. The PCR reaction mix contains genomic DNA having [...]

By |2018-02-27T08:43:02+00:00February 21st, 2018|Molecular Biology|Comments Off on Applications of Polymerase Chain Reaction (PCR)

Genetic Markers

In genetics, DNA marker is a gene or other fragment of DNA whose location in the genome is known. DNA markers are also known as molecular markers or genetic markers.It refers to any unique DNA sequence which can be used in DNA hybridization, PCR or restriction mapping to identify a particular sequence of DNA in [...]

By |2018-02-27T09:00:15+00:00February 20th, 2018|Molecular Biology|Comments Off on Genetic Markers

Southern blotting

Southern blotting is an example of RFLP (restriction fragment length polymorphism). It was developed by Edward M. Southern (1975). Southern blotting is a hybridization technique for identification of particular size of DNA from the mixture of other similar molecules. This technique is based on the principle of separation of DNA [...]

By |2018-02-27T09:08:53+00:00February 20th, 2018|Molecular Biology|Comments Off on Southern blotting

PCR (Polymerase Chain Reaction)

Polymerase chain reaction is a revolutionary method developed by Kary Mullis in the 1980s. PCR is based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand. Because DNA polymerase can add a nucleotide only onto a preexisting 3'-OH group, it needs a primer to [...]

By |2018-02-27T09:04:41+00:00February 20th, 2018|Molecular Biology|Comments Off on PCR (Polymerase Chain Reaction)

Bacterial identification through PCR technique

Bacteria is a prokaryotic organism having a large number of species. They are present in most of the habitats of earth. Some bacterias are useful while others are pathogenic and even threatful to life. Knowledge of the true bacterial species is fundamental for the effective utilization of that particular species . Most of the [...]

By |2018-02-27T08:32:29+00:00December 19th, 2016|Molecular Biology|Comments Off on Bacterial identification through PCR technique

News Updates

  • An MoU was signed between Orbit Biotech and Govt of Punjab during the Progressive Punjab Invest Summit 2015.
  • Registrations open for the biotech industrial training program starting April/May/June/July 2018. For registrations contact us at +91. 85 91 87 1105
error: Content is protected !!