Molecular Biology Protocols

Isolation of plasmid by alkaline lysis
Isolation of genomic DNA
Isolation of RNA from tissue
Organic extraction of DNA
Isolation of plasmid DNA by boiling lysis method and analysis by gel electrophoresis
Digestion of extracted plasmid
Ligation of digested product
Restriction Fragment Length Polymorphism
Analysis of nucleic acids by gel electrophoresis
Preparation of competent cells and transformation of E.coli
PCR amplification of extracted DNA fragment