Book Seat Now
Module DE
Book Seat Now
Module DE
Food Adulteration & Probiotic Development
Wine & Boza Fermentation & Enzymology
Duration: 8 Weeks
Timings: 4-5 hrs (Monday – Friday)
Eligibility: B.Sc./M.Sc. student of biotechnology/ life science or any scientific background
Status: Current/ Passout Eligible
Contents of the program
- Good Laboratory Practices (GLP)
- Principle & working of laboratory instruments
- Introduction about food adulteration & its types
- Checking adulteration in milk
- Checking adulteration in dairy products
- Checking adulteration in sweets
- Checking adulteration in Spices
- Checking adulteration in cereals
- Checking adulteration in oils
- Introduction to industrial microbiology
- Calculation techniques for industrial microbiology
- Introduction to sterilization techniques
- Media preparation (solid & liquid media)
- Dilution techniques for a sampling of air, water & soil
- Isolation of bacteria from air, water & soil
- Calculation of Colony Forming Units (CFU/ml)
- Isolation of microorganisms on selective media
- Identification of microorganisms using different staining methods
- Identification of microorganisms using motility methods
- Basic biochemical characterization – 1 of isolated microorganisms
- Preservation of microbial cultures by glycerol stock method
- A sampling of probiotic bacteria from food samples and isolation on specialized enriched media.
- Subculturing of microorganisms on specialized enriched media
- Bacterial measurements using microscopic techniques (micrometry)
- Partial Characterization – 2 of bacterial isolates using biochemical analysis (IMViC Tests)
- Microencapsulation of the probiotic cultures for use in functional foods & therapeutics
- Microbial production of crude bacteriocin
- To check the Antimicrobial Activity of crude bacteriocin
- Probiotic attribute analysis – Acid tolerance
- Probiotic attribute analysis – Bile tolerance
- Probiotic attribute analysis – Antimicrobial activity
- Principle & working of laboratory instrument
- Introduction to fermentation technology & fermenter designing
- Introduction to various types of wines
- Estimation of sugar content in juices
- Estimation of tartaric acid in juices
- Introduction to sterilization techniques
- Inoculation of wine
- Immobilization of fermentation cultures
- Estimation of the reducing sugar by DNSA method
- Estimation of ethanol by potassium dichromate method
- Estimation of alcohol by specific gravity method
- Introduction to enzyme technology
- Introduction & application to commercially important
- Preparation of media for enzyme production
- Dilution techniques for enzyme producer Boza
- Production of commercially important enzymes
- Screening of amylase-producing microorganisms
- Identification of different enzymes isolated from different sources. (Amylase, invertase & catalase)
- Screening of lipase-producing microorganisms.
- Preservation of microbial cultures by glycerol stock method
- Isolation of probiotic bacteria from milk & milk products
- Preparation of raw materials for Boza production
- Production of fermented probiotic product Boza
- Distillation of Wine produced
- Antimicrobial analysis of wine produced.
- Determination of Thermal Death Point (TDP) of fermentative microorganism.
- Determination of Thermal Death Time (TDT) of fermentative microorganisms.
- Probiotic attribute analysis – Antimicrobial activity
- To estimate the minimum inhibitory concentration (MIC) of enzymes products by microorganisms
- Session on CV drafting & personality development