Module D

Contents of the program

1. Good Laboratory Practices (GLP)
2. Principle & Working of Laboratory Instruments
3. Introduction to Industrial Microbiology
4. Calculation Techniques for Industrial Microbiology
5. Introduction to Sterilization Techniques
6. Media Preparation (Solid & Liquid Media)
7. Dilution Techniques for Sampling of Air, Water & Soil
8. Isolation of Bacteria from Air, Water & Soil
9. Calculation of Colony Forming Units (CFU/ml)
10. Isolation of Microorganisms on Selective Media
11. Identification of Microorganisms using Staining Methods
12. Identification of Microorganisms using Motility Methods
13. Basic Biochemical Characterization of Isolated Microorganisms
14. Preservation of Microbial Cultures by Glycerol Stock Method
15. Sampling of Probiotic Bacteria from Food Samples and Isolation on Enriched Media
16. Sub-culturing of Microorganisms on Specialized Enriched Media
17. Bacterial Measurements using Microscopic Techniques (Micrometry)
18. Partial Characterization of Bacterial Isolates (IMViC Tests)
19. Microencapsulation of Probiotic Cultures for Functional Foods & Therapeutics
20. Microbial Production of Crude Bacteriocin
21. Evaluation of Antimicrobial Activity of Bacteriocin
22. Probiotic Attribute Analysis – Acid Tolerance
23. Probiotic Attribute Analysis – Bile Tolerance
24. Probiotic Attribute Analysis – Antimicrobial Activity
25. Single Cell Protein (SCP) Production
26. Estimation of Protein Content in SCPs
27. Paper Chromatography
28. Phytochemical Analysis of Herbal Extract
29. Carbohydrates utilization test
30. Assessment of Microbial Growth
31. Lactophenol Cotton Blue Staining for Fungi
32. Biofilm Formation Assay
33. Determination of Minimum Inhibitory Concentration (MIC)
34. Determination of Minimum Bactericidal Concentration (MBC)
35. Session on CV drafting & Personality development