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Module E
Wine & Boza Fermentation & Enzymology

Duration: 4 Weeks
Timings: 4-5 hrs (Monday – Friday)
Eligibility: B.Sc./M.Sc. student of biotechnology/ life science or any scientific background
Status: Current/ Passout Eligible

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Contents of the program

  1. Good Laboratory Practices (GLP)
  2. Principle & working of laboratory instruments
  3. Introduction about fermentation technology & fermenter designing
  4. Introduction to various types of wines
  5. Estimation of sugar content in juices
  6. Estimation of tartaric acid in juices
  7. Introduction to sterilization techniques
  8. Inoculation of wine
  9. Immobilization of fermentation cultures
  10. Estimation of the reducing sugar by DNSA method
  11. Estimation of ethanol by potassium dichromate method
  12. Estimation of alcohol by specific gravity method
  13. Introduction about enzyme technology
  14. Introduction & application to commercially important enzymes products by microorganisms
  15. Preparation of media for enzyme production
  16. Dilution techniques for enzyme producer
  17. Production of commercially important enzymes
  18. Screening of amylase producing microorganisms
  19. Identification of different enzymes isolated from different sources. (Amylase, invertase & catalase)
  20. Screening of lipase producing microorganisms
  21. Production of enzymes using submerged fermentation and its crude extraction
  22. Preservation of microbial cultures by glycerol stock method
  23. Isolation of probiotic bacteria from milk & milk products
  24. Preparation of raw material for Boza production
  25. Production of fermented probiotic product Boza
  26. Distillation of Wine produced
  27. Antimicrobial analysis of wine produced.
  28. Determination of Thermal Death Point (TDP) of fermentative microorganism.
  29. Determination of Thermal Death Time (TDT) of fermentative microorganism.
  30. Probiotic attribute analysis – Antimicrobial activity
  31. To estimate minimum inhibitory concentration (MIC) of
  32. Boza
  33. DDPH Assey
  34. Ascorbic acid assay
  35. H2O2 scavanging activity
  36. Screening of proteases
  37. Preparation of hand sanitizer
  38. Preparation of vinegar
  39. Frap Assay
  40. Ascorbic Acid Assay
  41. Preparation of mocktails
  42. Preparation of kimchi
  43. Preparation sauerkraut
  44. Session on CV drafting & personality development
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