Module C

Contents of the program

1. Good Laboratory Practices (GLP)
2. Principle & working of laboratory instruments
3. Preparation of buffers & solutions
4. Basic calculation techniques
5. Introduction to sterilization techniques
6. Preparation of LB Media & inoculation
7. Isolation of genomic DNA by CTAB method
8. Preservation of nucleic acids
9. Preparation of TAE, Agarose gel and 6X gel loading buffer
10. Analysis of nucleic acids by gel electrophoresis
11. Organic extraction of DNA
12. Preparation of stock solutions for plasmid isolation
13. To study the role of various components used in isolation of plasmid
14. Isolation of plasmid by alkaline lysis
15. Preparation of competent cells
16. Preparation of IPTG, X-gal and ampicillin solution
17. Transformation of E.coli
18. Hands on handling thermo cycler & detection of specific gene through PCR
19. Detection of finger prints by I₂ method.
20. Quantification analysis of DNA.
21. Extraction of DNA from plants and fruits.
22. Venipunctures.
23. Isolation of DNA from plants, onions & bananas.