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Module E
Wine & Boza Fermentation & Enzymology

Duration: 4 Weeks
Timings: 4-5 hrs (Monday – Friday)
Eligibility: B.Sc./M.Sc. student of biotechnology/ life science or any scientific background
Status: Current/ Passout Eligible

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Contents of the program

  1. Good Laboratory Practices (GLP)
  2. Principle & working of laboratory instruments
  3. Introduction about fermentation technology & fermenter designing
  4. Introdution to various types of wines
  5. Estimation of sugar content in juices
  6. Estimation of tartaric acid in juices
  7. Introduction to sterilization techniques
  8. Inoculation of wine
  9. Immobilization of fermentation cultures
  10. Estimation of the reducing sugar by DNSA method
  11. Estimation of ethanol by potassium dichromate method
  12. Estimation of alcohol by specifi gravity method
  13. Introduction about enzyme technology
  14. Introduction & application to commercially important enzymes products by microorganisms
  15. Preparation of media for enzyme production
  16. Dilution techniques for enzyme producer
  17. Production of commercially important enzymes
  18. Screening of amylase producing microorganisms
  19. Identification of different enzymes isolated from different sources. (Amylase, invertase & catalase)
  20. Screening of lipase producing microorganisms
  21. Preservation of microbial cultures by glycerol stock method
  22. Isolation of probiotic bacteria from milk & milk products
  23. Preparation of raw material for Boza production
  24. Production of fermented probiotic product Boza
  25. Distillation of Wine produced
  26. Antimicrobial analysis of wine produced.
  27. Determination of thermal Death Point (TDP) of fermentative microorganism.
  28. Determination of Thermal Death Time (TDT) of fermentative microorganism.
  29. Probiotic attribute analysis – Antimicrobial activity
  30. To estimate minimum inhibitory concentration (MIC) of Boza
  31. DDPH Assey
  32. Ascorbic acid assay
  33. H2O2 scavanging activity
  34. Screening of proteases
  35. Preparation of hand sanitizer
  36. Preparation of vinegar
  37. Frap Assay
  38. Ascorbic Acid Assay
  39. Preparation of mocktails
  40. Preparation of kimchi
  41. Preparation sauerkraut
  42. Session on CV drafting & personality development
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