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Module AB
Food adulteration & Industrial Microbiology (2 Weeks)
Wine Fermentation & Enzymology (2 Weeks)

Duration: 4 Weeks
Timings: 4-5 hrs (Monday – Friday)
Eligibility: B.Sc./M.Sc. student of biotechnology/ life science or any scientific background
Status: Current/ Passout Eligible

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Contents of the Program

  1. Good Laboratory Practices (GLP)
  2. Principle & working of laboratory instruments
  3. Introduction about food adulteration & its types
  4. Checking adulteration in milk
  5. Checking adulteration in dairy products
  6. Checking adulteration in sweets
  7. Checking adulteration in spices
  8. Checking adulteration in cereals
  9. Checking adulteration in oils
  10. Introduction to industrial microbiology
  11. Calculation techniques for industrial microbiology
  12. Introduction to sterilization techniques
  13. Media preparation (solid & liquid media)
  14. Dilution techniques for a sampling of air, water & soil
  15. Isolation of bacteria from air, water & soil
  16. Calculation of Colony Forming Units (CFU/ml)
  17. Isolation of microorganisms on selective media
  18. Identification of microorganisms using different staining methods identification of microorganisms using motility methods
  19. Basic biochemical characterization – 1 of isolated microorganisms
  20. Preservation of microbial cultures by glycerol stock method
  21. Principle & working of laboratory instruments
  22. Introduction to fermentation technology & fermenter designing
  23. Introduction to various types of wine.
  24. Estimation of sugar content in juices
  25. Estimation of tartaric acid in juices
  26. Introduction to sterilization techniques
  27. Inoculation of wine
  28. Immobilization of fermentation cultures
  29. Estimation of the reducing sugar by DNSA method
  30. Estimation of ethanol by potassium dichromate method
  31. Estimation of alcohol by specific gravity method.
  32. Introduction about enzyme technology
  33. Introduction & application to commercially important enzymes products by microorganisms.
  34. Preparation of media for enzyme production
  35. Dilution techniques for enzyme producer sources. (Amylase, invertase & catalase).
  36. Preservation of microbial cultures by glycerol stock method.
  37. Screening of lipase producing microorganisms
  38. Identification of different enzymes isolated from different
  39. Screening of amylase-producing microorganisms
  40. Production of commercially important enzymes
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