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Module DE
Food Adulteration & Probiotic Development
Wine & Boza Fermentation & Enzymology

Duration: 8 Weeks
Timings: 4-5 hrs (Monday – Friday)
Eligibility: B.Sc./M.Sc. student of biotechnology/ life science or any scientific background
Status: Current/ Passout Eligible

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Contents of the program

  1. Good Laboratory Practices (GLP)
  2. Principle & working of laboratory instruments
  3. Introduction about food adulteration & its types
  4. Checking adulteration in milk
  5. Checking adulteration in dairy products
  6. Checking adulteration in sweets
  7. Checking adulteration in Spices
  8. Checking adulteration in cereals
  9. Checking adulteration in oils
  10. Introduction to industrial microbiology
  11. Calculation techniques for industrial microbiology
  12. Introduction to sterilization techniques
  13. Media preparation (solid & liquid media)
  14. Dilution techniques for a sampling of air, water & soil
  15. Isolation of bacteria from air, water & soil
  16. Calculation of Colony Forming Units (CFU/ml)
  17. Isolation of microorganisms on selective media
  18. Identification of microorganisms using different staining methods
  19. Identification of microorganisms using motility methods
  20. Basic biochemical characterization – 1 of isolated microorganisms
  21. Preservation of microbial cultures by glycerol stock method
  22. A sampling of probiotic bacteria from food samples and isolation on specialized enriched media.
  23. Subculturing of microorganisms on specialized enriched media
  24. Bacterial measurements using microscopic techniques (micrometry)
  25. Partial Characterization – 2 of bacterial isolates using biochemical analysis (IMViC Tests)
  26. Microencapsulation of the probiotic cultures for use in functional foods & therapeutics
  27. Microbial production of crude bacteriocin
  28. To check the Antimicrobial Activity of crude bacteriocin
  29. Probiotic attribute analysis – Acid tolerance
  30. Probiotic attribute analysis – Bile tolerance
  31. Probiotic attribute analysis – Antimicrobial activity
  32. Principle & working of laboratory instrument
  33. Introduction to fermentation technology & fermenter designing
  34. Introduction to various types of wines
  35. Estimation of sugar content in juices
  36. Estimation of tartaric acid in juices
  37. Introduction to sterilization techniques
  38. Inoculation of wine
  39. Immobilization of fermentation cultures
  40. Estimation of the reducing sugar by DNSA method
  41. Estimation of ethanol by potassium dichromate method
  42. Estimation of alcohol by specific gravity method
  43. Introduction to enzyme technology
  44. Introduction & application to commercially important
  45. Preparation of media for enzyme production
  46. Dilution techniques for enzyme producer Boza
  47. Production of commercially important enzymes
  48. Screening of amylase-producing microorganisms
  49. Identification of different enzymes isolated from different sources. (Amylase, invertase & catalase)
  50. Screening of lipase-producing microorganisms.
  51. Preservation of microbial cultures by glycerol stock method
  52. Isolation of probiotic bacteria from milk & milk products
  53. Preparation of raw materials for Boza production
  54. Production of fermented probiotic product Boza
  55. Distillation of Wine produced
  56. Antimicrobial analysis of wine produced.
  57. Determination of Thermal Death Point (TDP) of fermentative microorganism.
  58. Determination of Thermal Death Time (TDT) of fermentative microorganisms.
  59. Probiotic attribute analysis – Antimicrobial activity
  60. To estimate the minimum inhibitory concentration (MIC) of enzymes products by microorganisms
  61. Session on CV drafting & personality development
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