Module F

Contents of the program

1. Good Laboratory Practices (GLP)
2. Principle & working of laboratory instruments
3. Preparation of buffers & solutions
4. Basic calculation techniques
5. Introduction to sterilization techniques
6. Preparation of LB Media & inoculation
7. Isolation of genomic DNA by CTAB method
8. Preservation of nucleic acids
9. preparation of TAE, Agarose gel and 6X gel loading buffer
10. Analysis of nucleic acids by gel electrophoresis
11. Organic extraction of DNA
12. Preparation of stock solutions for plasmid isolation
13. To study the role of various components used in isolation of plasmid
14. Isolation of plasmid by alkaline lysis
15. Preparation of competent cells
16. Preparation of IPTG, X-gal and ampicillin solution
17. Transformation of E.coli
18. Hands on handling thermo cycler & detection of specific gene through PCR
19. Introduction to forensic science & DNA fingerprinting
20. Isolation of genomic DNA from human blood sample
21. Isolation of genomic DNA from hair
22. Isolation of genomic DNA from nail clipping sample
23. Preparation of master mix for PCR amplification
24. PCR amplification using random primers for RAPD
25. Preparation of TRI reagent & solutions for RNA isolation
26. DEPC treatment of the material required for RNA isolation
27. Isolation Of RNA from tissue
28. Restriction fragment length polymorphism (RFLP)
29. Detection of finger prints by 12 (Iodine) method
30. Isolation of protrins from blood and plants
31. Spectophotometric quantification of DNA, RNA & proteins.
32. Detection of blood groups.
33. Haemagglutination
34. SDS Page
35. Vein puncture to Extract blood.
36. Extraction of DNA from plants & fruits.
37. Session on CV drafting & personality development