Module DF

Contents of the program

1. Good Laboratory Practices (GLP)
2. Principle & working of laboratory instruments
3. Introduction about food adulteration & its types
4. Checking adulteration in milk
5. Checking adulteration in dairy products
6. Checking adulteration in sweets
7. Checking adulteration in spices
8. Checking adulteration in cereals
9. Checking adulteration in oils
10. Introduction to industrial microbiology
11. Calculation techniques for industrial microbiology
12. Introduction to sterilization techniques
13. Media preparation (solid & liquid media)
14. Dilution techniques for sampling of air, water & soil
15. Isolation of bacteria from air, water & soil
16. Calculation of Colony Forming Units (CFU/ml)
17. Isolation of microorganisms on selective media
18. Identification of microorganisms using different staining methods
19. Identification of microorganisms using motility methods
20. Basic biochemical characterization – 1 of isolated microorganisms
21. Preservation of microbial cultures by glycerol stock method
22. A sampling of probiotic bacteria from food samples and isolation on specialized enriched media.
23. Subculturing of microorganisms on specialized enriched media
24. Bacterial measurements using microscopic techniques (micrometry)
25. Partial Characterization – 2 of bacterial isolates using biochemical analysis (IMViC Tests)
26. Microencapsulation of the probiotic cultures for use in functional foods & therapeutics
27. Microbial production of crude bacteriocin
28. To check the Antimicrobial Activity of crude bacteriocin
29. Probiotic attribute analysis – Acid tolerance
30. Probiotic attribute analysis – Bile tolerance
31. Probiotic attribute analysis – Antimicrobial activity
32. Principle & working of laboratory instruments
33. Preparation of buffers & solutions
34. Basic calculation techniques
35. Introduction to sterilization techniques
36. Preparation of LB Media & inoculation
37. Isolation of genomic DNA by CTAB method
38. Preservation of nucleic acids
39. Preparation of TAE, Agarose gel and 6X gel loading buffer
40. Analysis of nucleic acids by gel electrophoresis
41. Organic extraction of DNA
42. Preparation of stock solutions for plasmid isolation
43. To study the role of various components used in the isolation of plasmid
44. Isolation of plasmid by alkaline lysis
45. Preparation of competent cells
46. Preparation of IPTG, X-gal and ampicillin solution
47. Transformation of E.coli
48. Hands-on handling Thermo cycler & detection of the specific gene through PCR
49. Introduction to forensic science& DNA fingerprinting
50. Isolation of genomic DNA from a human blood sample
51. Isolation of genomic DNA from hair
52. Isolation of genomic DNA from nail clipping sample
53. Preparation of master mix for PCR amplification
54. PCR amplification using random primers for RAPD
55. Preparation of TRI reagent & solutions for RNA isolation
56. DEPC treatment of the material required for RNA isolation
57. Isolation of RNA from tissue
58. Restriction fragment length polymorphism (RFLP)
59. Session on CV drafting & personality development